2 edition of Molecular and cytochemical characterization of an Arabidopsis thaliana meiotic mutant found in the catalog.
Molecular and cytochemical characterization of an Arabidopsis thaliana meiotic mutant
Brenda N Peirson
Written in English
|Statement||by Brenda N. Peirson|
|The Physical Object|
|Pagination||iv, 147 leaves :|
|Number of Pages||147|
Male meiosis in higher organisms features synchronous cell divisions in a large number of cells. It is not clear how this synchrony is achieved, nor is it known whether the synchrony is linked to the regulation of cell cycle progression. Here, we describe an Arabidopsis mutant, named tardy asynchronous meiosis (tam), that exhibits a phenotype of delayed and asynchronous cell divisions during. Homologous recombination, together with selection, laid the foundation for traditional plant breeding. The recombination process that takes place during meiotic cell division is crucial for the creation of novel variations of highly desired traits by breeders. Gaining control over this process is important for molecular breeding to achieve more precise, large-scale and quicker plant improvement.
Using Arabidopsis thaliana as a model, we outline two different methods, that is, one chemical and one environmental, to induce male meiotic restitution and high frequency 2n pollen grain formation. In addition, we provide a set of simple and straightforward techniques to characterize alterations in male meiotic cell division and gametophytic. The crossover distribution in meiotic tetrads of Arabidopsis thaliana differs from those previously described for Drosophila and Neurospora. Whereas a chi-square distribution with an even number of degrees of freedom provides a good fit for the latter organisms, the fit for Arabidopsis was substantially improved by assuming an additional set of crossovers sprinkled, at random, among those.
Identification of the AtABCG22 gene and atabcg22 mutant alleles. (a) AtABCG22 gene structure and insertional mutation sites of two atabcg22 alleles. Square boxes represent exons; black bars represent introns. Transposon insertion in atabcg22‐1 and T‐DNA insertion in atabcg22‐2 are indicated with triangles. (b, c) Transparency ratio of the atabcg22‐1 and atabcg22‐2 mutant plants: 5. Meiosis is often described as a special case of cell division since it differs from mitosis in having two nuclear divisions without an intervening S-phase. It will be of great interest to uncover what molecular mechanisms underlie these special features of meiosis. We previously reported that the tardy asynchronous meiosis (tam) mutant of Arabidopsis (Arabidopsis thaliana) is slower in.
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In Arabidopsis, a mutant (atk1, for Arabidopsis thaliana kinesin1) was found to have greatly reduced male fertility and mutant male meiocytes form abnormal meiotic spindles (Chen et al., ).
The atk1 mutant meiotic spindles have unfocused poles, Cited by: Cytological characterization of four meiotic mutants of Arabidopsis isolated from T-DNA-transformed lines Another mutant is defective in meiotic cell cycle control and undergoes a third.
Recent studies of meiotic recombination in the budding yeast and the model plant Arabidopsis thaliana indicate that meiotic crossovers (COs) occur through two genetic pathways: the interference-sensitive pathway and the interference-insensitive pathway.
However, few genes have been identified in either pathway. Here, we describe the identification of the PARTING DANCERS (PTD) Cited by: Characterization of the AtFANCD2 Gene and Mutant Alleles.
We identified an Arabidopsis gene encoding a protein with 25% identity (and 44% similarity) to human FANCD2 by homology search and named it AtFANCD2 (At4g). Cloning and sequencing of the cDNA revealed that the gene comprises 31 exons and its open reading frame is base pairs in length (GenBank Cited by: Fluorescence microscopy was used to study meiosis in microsporocytes from wild‐type Arabidopsis thaliana and a T‐DNA‐tagged meiotic mutant.
Techniques for visualizing chromosomes and β‐tubulin in other plant species were evaluated and modified in order to develop a method for analyzing meiosis in A. thaliana anthers. Like most dicots, A. thaliana microsporocytes undergo simultaneous Cited by: Arabidopsis thaliana has proven a powerful system for developmental genetics, but identification of gametophytic genes with developmental mutants can be complicated by factors such as gametophyte-lethality, functional redundancy, or poor penetrance.
These issues are exemplified by the Plant Intracellular Ras-group LRR (PIRL) genes, a family of nine genes encoding a class of leucine-rich.
Bleuyard JY, Gallego ME, White CI () Meiotic defects in the Arabidopsis rad50 mutant point to conservation of the MRX complex function in early stages of meiotic recombination.
Chromosoma – PubMed CrossRef Google Scholar. In plants, whole-genome doubling (polyploidization) is a widely occurring process largely contributing to plant evolution and diversification.
The generation and fusion of diploid gametes is now considered the major route of plant polyploidization. The parallel arrangement or fusion of meiosis II MII spindles (ps) is one of the most frequently reported mechanisms generating triploid offspring. Arabidopsis thaliana UBC implication of error-free DNA damage tolerance and Lyslinked polyubiquitylation in plants.
Plant Molecular Biol – Wen R, Torres-Acosta JA, Pastushok L, Lai X, Pelzer L, Wang H, Xiao W. Arabidopsis UEV1D promotes Lysinelinked polyubiquitination and is involved in DNA damage response. Coram, J W, Harding, A J, Stamey, R. T, and Rundle, S J. () Characterization of DNA sequences encoding a novel isoform of the 55 kDa B regulatory subunit of the type 2A serine/threonine phosphatase of Arabidopsis thaliana Plant Mol.
B – Plant Material and Mutant Isolation. All Arabidopsis thaliana plants used in this study were in the Landsberg erecta background.
The seeds were pregerminated on MS agar plates (Murashige and Skoog, ) with or without 50 μg/mL kanamycin at 22°C under a h-light/8-h-dark cycle. The plants were grown in soil at 22°C under the same light. The HEM Lines: A New Library of Homozygous Arabidopsis thaliana EMS Mutants and its Potential to Detect Meiotic Phenotypes FRONT PLANT SCI AT1G AT1G AT1G AT2G AT3G AT3G AT3G AT3G AT4G AT4G AT5G AT5G In this paper, we describe the cloning of the MS5 gene, a gene essential for male fertility in previously defined the MS5 locus by characterizing an EMS‐induced allele, ms5– identified a new allele of MS5 (ms5–2) that was T‐DNA‐generated and used the T‐DNA tag to clone the cing of mutant and wild‐type alleles together with complementation of the.
Puromycin-sensitive aminopeptidases (PSAs) participate in a variety of proteolytic events essential for cell growth and viability, and in fertility in a broad range of organisms. We have identified and characterized an Arabidopsis thaliana mutant (mpa1) from a pool of T-DNA tagged lines that lacks PSA activity.
This line exhibits reduced fertility, producing shorter siliques (fruits) bearing a lower number of. Molecular Characterization of the Transformants. Transformation of Arabidopsis thaliana was performed as described (Clough and Bent, ).
Due to the sterility of the spo11 mutants, heterozygous plants had to be used for the transformation. For genotyping of spo, DNA was extracted from a small leaf of transformants grown in soil.
A meiotic time-course for Arabidopsis thaliana. Sexual Plant Reproduction. ; 16 (3)– doi: /s Couteau F, Belzile F, Horlow C, Grandjean O, Vezon D, Doutriaux MP. Random chromosome segregation without meiotic arrest in both male and female meiocytes of a dmc1 mutant of Arabidopsis.
Plant Cell. To identify strategies which may be used to reduce shatter, either by conventional breeding programmes or by genetic engineering, we have examined fruit development in oil‐seed rape (Brassica napus), and in the related B.
juncea and Arabidopsis, using a combination of cytological, cytochemical and molecular techniques. We report here on the. Arabidopsis thaliana (Thale cress) Q9LNT AT1G Search in QuickGO: Arabidopsis thaliana (Thale cress) O AT2G Search in QuickGO: Arabidopsis thaliana (Thale cress) O AT2G Search in QuickGO: Arabidopsis thaliana (Thale cress) O AT2G Search in QuickGO: Arabidopsis thaliana (Thale cress.
Isolation and Characterization of a Male Meiotic Mutant. Arabidopsis lines carrying Dissociation (Ds) insertions were generated and screened for mutant plants that exhibit defects in fertility and meiosis.
Progeny of one line (Y) segregated for fertile and sterile plants with a ratio of ∼, suggesting that the parental plant was heterozygous for a recessive mutation. Zwickau’s graduate research focused on the molecular and cytochemical characterization of an Arabidopsis thaliana meiotic mutant.
She has directed student research involving plant enzymes and pigments. Arabidopsis male meiosis yields four haploid cells organized as a balanced tetrad ().The pp2ab′αβ double mutant produced balanced tetrads (27%), unbalanced tetrads (%), triads (%), and polyads (%; Fig.
1E), indicating defects in chromosome segregation and/or meiotic reproduction also was affected in the pp2ab′αβ double mutant, as a reciprocal .In Arabidopsis, the expression of many meiotic genes such as AtDMC1 [9, 10], SDS, MMD1, and RCK is highly regulated.
Studying the commonness and distribution of CREs in the promoters of coexpressed genes can help facilitate the identification of signaling networks in specific cell types (e.g., [ 14 – 17 ]). To follow the dynamics of meiosis in the model plant Arabidopsis, we have established a live cell imaging setup to observe male meiocytes.
Our method is based on the concomitant visualization of microtubules (MTs) and a meiotic cohesin subunit that allows following five cellular parameters: cell shape, MT array, nucleus position, nucleolus position, and chromatin condensation.